Delivery by Dendritic Mesoporous Silica Nanoparticles Enhances the Antimicrobial Activity of a Napsin?Derived Peptide Against Intracellular Mycobacterium tuberculosis
نویسندگان
چکیده
Tuberculosis remains a serious global health problem causing 1.3 million deaths annually. The causative pathogen Mycobacterium tuberculosis (Mtb) has developed several mechanisms to evade the immune system and resistances many conventional antibiotics, so that alternative treatment strategies are urgently needed. By isolation from bronchoalveolar lavage peptide optimization, new antimicrobial named NapFab is discovered. While showing robust activity against extracellular Mtb, of intracellular bacteria limited due low availability. loading onto dendritic mesoporous silica nanoparticles (DMSN) as carrier system, cellular uptake, consequently antimycobacterial Mtb significantly enhanced. Furthermore, using lattice light-sheet fluorescence microscopy, it can be shown gradually released DMSN inside living macrophages over time. electron microscopy tomography, demonstrated loaded stored in vesicular structures proximity mycobacterial phagosomes cells, but typically not direct contact with bacteria. Based on combination functional live-cell imaging analyses, hypothesized after being able enter bacterial phagosome gain access bacilli.
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ژورنال
عنوان ژورنال: Advanced Healthcare Materials
سال: 2021
ISSN: ['2192-2640', '2192-2659']
DOI: https://doi.org/10.1002/adhm.202100453